Advances in Enzymology and Related Areas of Molecular by Alton Meister

By Alton Meister

Advances in Enzymology and comparable components of Molecular Biology is a seminal sequence within the box of biochemistry, delivering researchers entry to authoritative stories of the newest discoveries in all components of enzymology and molecular biology. those landmark volumes date again to 1941, offering an unequalled view of the ancient improvement of enzymology. The sequence bargains researchers the newest realizing of enzymes, their mechanisms, reactions and evolution, roles in complicated organic procedure, and their software in either the laboratory and undefined. every one quantity within the sequence good points contributions by way of major pioneers and investigators within the box from worldwide. All articles are rigorously edited to make sure thoroughness, caliber, and clarity.

With its wide selection of subject matters and lengthy old pedigree, Advances in Enzymology and comparable components of Molecular Biology can be utilized not just via scholars and researchers in molecular biology, biochemistry, and enzymology, but in addition by way of any scientist drawn to the invention of an enzyme, its homes, and its purposes.

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Catravas and Anker (303) 1 recently reaffirmed their original findings when they reported that both mitochondria and a soluble fraction were required for incorporation of acetate into the fatty acids of rat liver (46). They claimed, furthermore, that some of the lipogenic enzymes were extracted from mitochondria when these granules were prepared in electrolyte-containing solutions, and implied that this phenomenon might explain the discrepancies observed in the localization of the enzymes involved in fatty acid formation.

Since diglycerides and phosphatidic acid were observed to stimulate the incorporation of phosphorylcholine into lecithin, these compounds were considered to be intermediates in the formation of lecithin. Prior to the work of Rodbell and Hanahan, Kennedy (310) had reported that choline was incorporated into mitochondrial lecithin by a pathway that did not involve phosphorylcholine. This finding was admitted to be incorrect, however (311), and in subsequent work Kennedy and his co-workers were able to show that the synthesis of lecithin from phosphorylcholine involved its reaction with C T P to form cytidiriediphosphocholine (31 1).

This microsomal stimulation can be either enzymic or nonenzymic. Whether microsomes exert an effect on phosphorylation, however, is another matter. Siekevitz (98) observed no such stimulation although oxygen uptake was increased almost two-fold by microsomes. On the other hand, early experiments by Lehninger (119) indicated that microsomes may be involved in oxidative phosphorylation. He observed that DPNH added to isolated mitochondria under isotonic conditions was oxidized and phosphate was esterified, but that when the granules were pretreated 30 WALTER C.

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